Help disrupting yeast wall

Tom Dougherty Doughert at synapse.bms.com
Fri May 23 08:29:58 EST 1997

In article <Pine.A32.3.91.970522141410.22149A at alboran.ualm.es>, Horacio Cano <hcano at alboran.ualm.es> says:

>        I'm starting a study in wich I need to disrupt a yeast wall, and 
>I've several problems with it.
>        I would be very grateful if someone gave me information about how to
>do it, I mean, some bibliography or some advices of someone who has 
>worked with yeasts. 
      Is zymoliase worth for my purpose and where can I find it cheap? 
>Do you know some protocol to isolate some enzime to obtain protoplasts? 
>Does B-glucoronidase really works? Phisic breaking methods are enough?
>Must I use both mechanic and enzimatic methods?
>        Thank you in advance.

>                                        hcano at alboran.ualm.es
There is a relatively inexpensive device called a "Bead Beater" made by
a company called Biospec in Oklahoma.  We have had excellent results 
breaking many difficult gram positives and yeast with the device.  It uses
shearing action, so it cannot be used to isolate DNA, but it appears to 
work well for many enzymes.  Comes with a cooling jacket that you fill with
ice and water.  Email me if you need details.
Thomas J. Dougherty, Ph.D.
Dept. of Microbiology
Bristol-Myers Squibb Co.
Doughert at synapse.bms.com

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