In article <5lt2r7$lca$1 at news2.xs4all.nl>, hwilke at xs4all.nl (Hans Wilke) says:
>>For an experiment we need to make competent streptococci
>(S. pneumoniae, S. mutans). There are plenty of protocols on
>how to make E.coli competnet cells but I could not find any on
>streps. Any help is greatly appreciated.
>>Hans Wilke (hwilke at cryogen.com)
> Competance protocols in streptococci vary with the organism. For S.
pneumoniae, there is a newly described peptide (CSP-1) that is used with
the R6 strain to induce competance (see PNAS 92:11140, 1995). For S.
mutans (gordonii), horse serum is used; don't remember the percentage.
This undoubtedly contains some factor (peptide??) that induces competance.
Some streptococci cannot currently be made competant (e.g.,S. pyogenes).
Tom Dougherty
