How to make competent streptococci

Tom Dougherty Doughert at synapse.bms.com
Fri May 23 08:22:08 EST 1997

In article <5lt2r7$lca$1 at news2.xs4all.nl>, hwilke at xs4all.nl (Hans Wilke) says:
>For an experiment we need to make competent streptococci 
>(S. pneumoniae, S. mutans). There are plenty of protocols on 
>how to make E.coli competnet cells but I could not find any on 
>streps. Any help is greatly appreciated.
>Hans Wilke (hwilke at cryogen.com)
 Competance protocols in streptococci vary with the organism.  For S.
pneumoniae, there is a newly described peptide (CSP-1) that is used with
the R6 strain to induce competance (see PNAS 92:11140, 1995).  For S.
mutans (gordonii), horse serum is used; don't remember the percentage.
This undoubtedly contains some factor (peptide??) that induces competance.
Some streptococci cannot currently be made competant (e.g.,S. pyogenes).
Tom Dougherty

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