Need B-lactamase plasmid

Karl Fischer tyr-2 at bones.biochem.ualberta.ca
Sun May 18 12:22:36 EST 1997

In article <5ln419$4es$3 at daily-planet.nodak.edu>,
chambers at plains.nodak.edu (Michael A Chambers) wrote:

> Hello everyone,
> I would like to obtain a high-copy number plasmid encoding B-lactamase.  I
> realize that many plasmids utilize this as a transfection marker.
> However, I need a construct driven by a eukaryotic promoter (e.g., a
> plasmid encoding B-lactamase driven by the cytomeglovirus promoter.)
> Thank you very much for your help.
> Mike

Ummm...excuse me Mike....are you looking for beta-lactamase or
beta-galactosidase?? The former is the enzyme which breaks down
penicillin/ampicillin/carbenicillin to select for bacterial transformants
while the latter is best known (in mol bio) for conversion of X-gal into
its blue colored product as employed in blue-white discrimination in
bacterial colony selection or as a transfection control for eukaryotic

Plasmids with beta-galactosidase under control by CMV/SV40/LTR promoters
are as common as snowflakes on a January morn in Alaska; if someone at
your university doesn't have one (which would be strange indeed) then
there is a number of companies which sell them (Pharmacia, Stratagene,
Promega, etc.).

Karl the hepB guy

Karl Fischer
tyr-2 at bones.biochem.ualberta.ca

More information about the Microbio mailing list

Send comments to us at biosci-help [At] net.bio.net