TICKS AND LYME DISEASE: HELP WANTED

[Dr. Duncan Clark]

In article <5lf1ge$h3f$1 at gerry.cc.keele.ac.uk>, DR Hulse
<p6r38 at cc.keele.ac.uk> writes
>An immunofluorescence assay, and a PCR technique, however i cannot find a
>source of the nerssecary antibodies, or of the relevant primer ( the primer
>is the B. burgdorferi flagellin gene). I would be extremely grateful if
>anyone who knows of a source of these products  would contact me either via
>the newsgroups or by E-MAILing me on P6R38 at cc.keele.ac.uk.

I would suggest that you go to Genbank and pull every flagellin gene
sequence for Borrelia burgdorferi, align them and design appropriate
primer sets for conserved regions. A quick look at Genbank gave a large
list of deposited sequences including X15660, X15661, X14841 etc. I
searched by gopher to gopher.nih.gov.

Duncan 


-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288
http://www.dnamp.com
http://www.genesys.demon.co.uk