anaerobic media database

ngw at bng.nl ngw at bng.nl
Thu May 1 19:18:20 EST 1997

To All,

In the field of clinical and dairy anaerobic microbiology a large
number of selective isolation media are used. Some may be really
selective, some may be relatively non-selective. It seams that each
laboratory is using their own set of media. Unfortunately, this makes
comparing data rather difficult, if not impossible.
I am currently making a kind of database of media currently used for
isolation of anaerobic bacteria from clinical and dairy sources. Using
this database we might finally end up with a number of much used and
reliable isolation media. 
Therefore I would like to ask those who are working with anaerobes, if
they would mail or fax me the following information:
- which media are being used (an abbreviation could be used if it is a
medium described in the literature)
- if the media are prepared using a special in-house modification, a
description of the modifications
- known selectivity of the media (if published only a reference),
especially known contaminating flora. Only a very limited number of
publications actually describe the selectivity of a certain medium
- use of the media; i.e. human intestinal/human vaginal/rat
intestinal/dairy starters a.s.o.
- average bacterial numbers isolated on the media (if available)

I am looking for the following bacterial groups:
total anaerobes
clostridia total
	Cl. perfringens
	Cl. difficile
anaerobic streptococci
megasphaerae and veillonellae
	B. fragilis-group
other groups when available

So far I have included over 60 media from >100 publications.

The results will be published on paper and/or electronically. All
participants will receive a paper copy of the first version of the

Hopefully awaiting....

Ralf Hartemink
Intestinal microbiology group
Dept Food Microbiology
Wageningen Agricultural University
PO Box 8129
6700 EV Wageningen
The Netherlands
fax +31-317-484893
e-mail : ralf.hartemink at algemeen.lenm.wau.nl

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