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PCR and errors

JOHNNY TALAVERA johnnytquest at webtv.net
Tue Jun 17 23:20:48 EST 1997


I am using PCR to clone out a gene from a bacterial virus gene. I have
mutant variants of the gene which I would like to clone out for DNA
sequencing. How do I minimize introducing errors when cloning out the
gene from the phage to the sequehcing or expression vector? Please send
me suggestions and references at rinforma at caribe.net with my name on the
subject line. Thanks in advance. DrBob in PR.
Sincerely,                                                      
  Dr Bob in PR
  rinforma at caribe.net
   please place my name in the subject line.
    FAX: 787-740-4300
     Phone: 787-798-3001
UCC SCHOOL OF MEDICINE
DEPT OF MICROBIOLOGY
P.O. BOX 60-329
BAYAMON, PR 00960
                                     































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