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Curing plasmids with coumermycin

Dr Martin Goldberg mdg at leicester.ac.uk
Wed Jun 11 05:04:02 EST 1997


As a result of my enquiry regarding the curing of a  plasmid from my
strain, I tried using a sub-inhibitory concentration of coumermycin
and obtained a >70% cure-rate. The plasmid was different to the
pBR322-based plasmid described in my earlier entry; this one is a low
copy-number derivative of pSC101, but the outcome was the same. 
*****************************************
  Dr Martin Goldberg,                   
  Dept. of Microbiology and Immunology, 
  University of Leicester,              
  University Road,                      
  LEICESTER.                             
  LE1 9HN                               
  UK                                    
  Tel. +44 (0)116-252 3017              
  Fax. +44 (0)116-252 5030              
  E-mail mdg at le.ac.uk                   
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