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Yeast ADH2 promotor

J HAARHOFF, MIKROBIOLOGIE, TEL 4867 9152253 at MATIES.SUN.AC.ZA
Wed Jun 4 12:14:29 EST 1997


Hi everyone

I have a unusual situation.

I transformed a recombinant gene to S.cerevisiae, but found that the enzyme  
is only produced on solid media, NOT liquid media!

The media I use is SC-ura  (C-source: 0.8% glucose) and SC-ura (C-source 
Gliserol/EtOH) 
The recombinant gene is under control of the ADH2 promotor.

Has anyone had such a situation before, could ne1 please suggest a few 
possible explanations.

This work was supposed to complete my M.Sc but now it is slowing me down so 
that i may not finish in time.

Thanx

Jacques Haarhoff




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