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Curing plasmids from a strain

Dr Martin Goldberg mdg at leicester.ac.uk
Tue Jun 3 10:25:13 EST 1997


I have a strain with a pBR322-based plasmid and I want to cure the
strain of this plasmid. Is there any way of achieving this without
using agents such as ethidium bromide? I am considering competing-out
the plasmid by transforming the strain with pBR322 or one of its 
other derivatives and selecting for this latter plasmid. Has anybody got an
opinion on this approach or are there any more elegant methods? 
*****************************************
* Dr Martin Goldberg,                   *
* Dept. of Microbiology and Immunology, *
* University of Leicester,              *
* University Road,                      *
* LEICESTER.                            *
* LE1 9HN                               *
* UK                                    *
* Tel. +44 (0)116-252 3017              *
* Fax. +44 (0)116-252 5030              *
* E-mail mdg at le.ac.uk                   *
*****************************************



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