Pre-reduced media preparation

Frederick W. Hyde fredhyde at citilink.com
Tue Jul 29 16:38:00 EST 1997

Nisha Singh wrote:
 Cooling the media under
> nitrogen gas after boiling has also been unsuccessful. Is anyone able to
> help us? Thank you

How are you sparging with nitrogen? In chamber? When growing oral
spirochetes, we used the method you did with excellent success. All
steps of boiling and cooling were performed while sparging with
nitrogen, then the containers were sealed and autoclaved in a press. How
much resazurin are you using?


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