In article <33C1186D.6672 at ucsd.edu>, britchings at ucsd.edu wrote:
> Does anyone know if DMSO (7% v/v) works as well as glycerol (20% or
> thereabouts, final concentration) for storing frozen (-80C) bacterial
> stocks (gram negative, mostly E. coli)? Thanks, Bruce
>britchings at ucsd.edu
Both methods seem to work fine from my experience. One consideration might
be the toxicity of DMSO. Another consideration might be that glcerol has a
negative effect on the competence of E.coli cells (Inoue et al, ).
Thomas
--
Thomas Urbig
Urbig at biokemi.su.se
