humic acide

Gina Berardesco gbe392 at merle.acns.nwu.edu
Sun Jul 20 14:56:42 EST 1997

µ¡¤fÃ䪺¤p×^×^ (dodo.bbs at flight.bio.ncku.edu.tw) wrote:
> When i extracted DNA of bacteria from soil,there was a lot of humic acide
> in DNA extraction.And humic acide will affact PCR(polymerase cycle reaction).
> How should i do?Please tell me.
> thanks!

The best way to remove humics is to gel purify your DNA on an agarose gel.
You should see the humics running ahead of your DNA.


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