µ¡¤fÃ䪺¤p×^×^ (dodo.bbs at flight.bio.ncku.edu.tw) wrote:
> When i extracted DNA of bacteria from soil,there was a lot of humic acide
> in DNA extraction.And humic acide will affact PCR(polymerase cycle reaction).
> How should i do?Please tell me.
> thanks!
The best way to remove humics is to gel purify your DNA on an agarose gel.
You should see the humics running ahead of your DNA.
-gb
