IUBio

humic acide

Zhongtang Yu zyu at UNIXG.UBC.CA
Sat Jul 19 23:07:25 EST 1997


You may try to purify your DNA prep with some spun-columns, like Chroma
Spin 400 (from Clontech) or Wizard (from Promega), or Sephedax columns.

Zhongtang Yu, Ph.D.
Microbiol., UBC.

On 19 Jul 1997, =B5=A1=A4f=C3=E4=AA=BA=A4p=D7^=D7^ wrote:

> When i extracted DNA of bacteria from soil,there was a lot of humic acide
> in DNA extraction.And humic acide will affact PCR(polymerase cycle reacti=
on).
> How should i do?Please tell me.
> thanks!
>=20
>=20
> --
> =A1=B0=A8=D3=B7=BD:=A6=A8=A4j=AD=B8=B5=BE =A1=BB=A7=DA=AA=BA=A5D=A4H dodo=
 =A8=D3=A6=DB:140.116.25.21
> =1B[33m=A1=C0=1B[35mOrigin:=1B[1;31m=A6=A8=1B[1;33m=A4j=1B[32m=A5=CD=1B[0=
;34m=AA=AB=1B[m=1B[1;37m(flight.bio.ncku.edu.tw)=1B[m
>=20
>=20




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