IUBio

DMSO vs glycerol

Ivano de Filippis ivano at ALPHA.INCQS.FIOCRUZ.BR
Wed Jul 9 08:24:10 EST 1997


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At 16:25 07/07/97 +0000, you wrote:
>Does anyone know if DMSO (7% v/v) works as well as glycerol (20% or
>thereabouts, final concentration) for storing frozen (-80C) bacterial
>stocks (gram negative, mostly E. coli)? Thanks, Bruce
>britchings at ucsd.edu
>
>

Have you tried with Skim Milk (DIFCO) at 10% (w/v)? It works very well also.

Ivano



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Ivano de Filippis
Fundacao Oswaldo Cruz - FIOCRUZ
Instituto Nacional de Controle de Qualidade em Saude - INCQS
Depto. de Microbiologia
Lab. de Materiais de Refer=EAncia
Av. Brasil, 4365 - Manguinhos
Rio de Janeiro - 21045-900 - BRASIL
Tel.: 55-21-598-4290/4291/4292/4293
FAX: 55-21-290-0915
E-mail: ivano at alpha.incqs.fiocruz.br

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