Bacterial enumeration from coloidal silica

Rocky Baker bakerr at ucs.orst.edu
Wed Jul 9 18:19:51 EST 1997

Can anyone suggest a technique to enumerate bacteria in a colloidal silica 
solution.  The solution is composed of colloidal silica (15 nm particle 
size) in water containing an amine.  The pH is 10 and the solution has a 
susdsy appearance when shaken.  The concentration of silica is unknown to 
me but the solution is slightly opaque.  We are interested in techniques 
to enumerate any bacteria present in the slurry using epifluorescence or 
culturing methods. The solution will rapidly clog a 0.2 u membrane filter.

Rocky Baker

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