IUBio

G+ DNA extraction help

Mark Fuller mark_fuller at qmgate.anl.gov
Fri Feb 28 16:43:29 EST 1997


Collegues,

I would like to extract total DNA from soil slurry reactor and I 
especially interested in the gram-positive segment of the microbial 
community.  Is it known whether DNA is harder to extract from 
gram-positive organisms and spores, or will normal procedures work 
well?  Do you recommend lysozyme use?  

Any references would be much appreciated.

Thanks in advance,

Mark Fuller, Ph.D.
Argonne National Laboratory
mark_fuller at qmgate.anl.gov



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