Collegues,
I would like to extract total DNA from soil slurry reactor and I
especially interested in the gram-positive segment of the microbial
community. Is it known whether DNA is harder to extract from
gram-positive organisms and spores, or will normal procedures work
well? Do you recommend lysozyme use?
Any references would be much appreciated.
Thanks in advance,
Mark Fuller, Ph.D.
Argonne National Laboratory
mark_fuller at qmgate.anl.gov