unknown anaerobe

Dr Koen A.L. De Smet k.desmet at ic.ac.uk
Tue Feb 25 10:47:39 EST 1997

Dear Ralf,

Being a molecular biologist, I suggest you do a PCR on part of the 16S 
rRNA gene and sequence the PCR product. Compare the sequence to the 
DNA database and yuo will at least find the genus, and probably the 
species (if it is a known species). I have used this technique 
succesfully on several species, including anaerobes cultured from 
faeces. Contact me by email if you would like to receive more 
information on it.

Koen De Smet
k.desmet at ic.ac.uk

Ralf Hartemink wrote:
> Hi,
> We have recently isolated an anaerobic bacterium from a number of human
> faecal samples. So far we have not been able to identify the genus or
> species.
> the cells are mono or duplococci 2-5 um diameter
> anaerobic
> aerotolerant (up to 1 h)
> able to grow at an initial pH of 5.0
> yellow to white colonies
> vancomycin resistant
> katalase/oxidase negative
> Gram negative
> produce some gas
> final pH in RCM 4.2-4.5
> do not require fermentable carbohydrate for growth
> The cells closely resemble Megasphaera species, but as they grow at pH
> 5.0 are not one of the known species. The other characteristics resemble
> Sarcina, but the cells are always mono- or duplococci. Other Gram
> negative anaerobic cocci fail to grow at pH 5.0 or do not confirm to
> morphology or other characteristics.
> The strains are isolated from several human samples in logN of 3-6/gram
> wet faeces.
> Any help is appreciated !
> Ralf Hartemink
> Ralf.Hartemink at algemeen.lenm.wau.nl or ngw at teleoffice.nl
> Wageningen Agricultural University
> Section Food Microbiology
> Intestinal Microbiology Group

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