You have to be careful with the fluorescein produced by Pseudomonads. The
degree of fluoresence of extracts will drop dramatically up addition of
iron. (Try it).
There is a company in the US that produces a photobacterium based assay for
pollutants.
In article <2EDD5B203F0 at server1.rtc-athlone.ie>,
MDAVOREN POST GRAD <mdavoren at SERVER1.RTC-ATHLONE.IE> wrote:
>Hi there,
> I am currently working with Pseudomonas fluorescens as
>part of my Master's Degree research. My objective is to test a chemical on
>the bacteria using the decrease in fluorescence as a measure of
>toxic response. Has anyone performed this test procedure as i would
>love to hear from them and would be extremely grateful if anyone
>could recommend some literature to read up on this subject. I also
>envisage using the marine photobacteria - Photobacterium
>phosphorens, so any info. on this would be also welcome.
>>> Thanks in advance,
>>> Maria Davoren.
> Toxicology Unit,
> Athlone RTC,
> Co. Westmeath, Ireland.
>mdavoren at server1.rtc-athlone.ie
--
Wayne Lee Forday, Biotechnology Department
Ngee Ann Polytechnic, Singapore
fwl at np.ac.sg
Fax:467-9109
