Screening for Amp(s) bacteria.

Szatmari George szat at ERE.UMontreal.CA
Thu Feb 13 14:10:07 EST 1997

In article <Pine.SOL.3.91.970211131631.4808B-100000 at howard> Rafael Maldonado <rafael at howard.genetics.utah.edu> writes:
>Hello all,
>I am searching for a method, involving starch, iodine and penicillin, to 
>screen for Amp(s) clones of bacteria. I know the method exists, but I 
>cannot find the reference in Medline. The screening is based in different 
>color of the colonies growing in solid medium contaning such ingredients.
>Any other reference, using other method, to screen for Amp(s) E. coli 
>clones will be wellcome.

Dear Rafa,

The reference is Boyko and Ganshow 1982, Anal. Biochem 122: 85-88

I've tried it and it works, as long as your plates don't contain
too many colonies.

George Szatmari
Dept de microbiologie, Universite de Montreal

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