IUBio

Staphylococcus aureus lysis

Daniel Larrimore dlarrimo at indiana.edu
Tue Feb 4 00:48:40 EST 1997


Keith Warner (kwarner at unccvm.uncc.edu) wrote:
> Here is the problem.  I am working with several isogenic strains of S.
> aureus.  One of the strains will often lose turbidity after 3 to 4 hours
> growth in broth and eventually become completely clear.  This clearing
> occurs only after inoculation of fresh broth with an overnight broth
> culture--never happens in broth when inoculating from a plate. Addition of
> the lysate to spread plates of S. aureus, E. coli, and B. subtilis produce
> 'plaques'. Filtration of the lysate with a 0.45 micron pore filter
> eliminates plaque formation.  What's the story?
 
It does sound like Bdellovibrio, but they usually prefer Gram negative 
bacteria.  Do you see any small colonies in the plaques which would be 
typical of a phage plaque, or if it is indeed Bdellovibrio, a simple Gram 
stain of the culture should reveal Gram negative contamination.  

						Dan Larrimore



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