Mark Wolcott wrote,
<Does anyone know of a commercial source for Mycoplasma media? I'm
particularly interested in SP4 or Jordan's formulations.>
Difco sells PPLO media for the growth of "pleuro-pneumonia-like
organisms," i.e., mycoplasma. However, it doesn't say anything about SP4
or Jordan's formulations (and alas, I'm not familiar with those either,
not having worked with mycoplasma myself). I can't judge, therefore, if
this is the media you want, but perhaps you can from the recipes:
Per Liter of DI H20:
PPLO Agar:
Beef Heart Infusion: 50 g
Peptone: 10 g
NaCl: 5 g
Agar: 14 g
PPLO Broth Media:
Formulations same as above, minus the agar. Available with and without
crystal violet.
PPLO Serum fraction (sterile solution of partially purified serum
fraction required for growth of mycoplasma)
Well, I hope this helps you a little.
Infectionately,
Yersinia.
"Van der waals with boughs of holly..."
