Sorry, got it wrong. Sold via sensititre/almar; accumed international
co, westlake ohio.
Regards
Gerhard
Regarding my statement that it is resazurin, I found the supporting
reference :
Evaluation of bacterial survival and phagocyte function with a
fluorescence-based microplate assay
Shiloh MU, Ruan J, Nathan C
INFECTION AND IMMUNITY
65: (8) 3193-3198 AUG 1997
Author: cnathan at med.cornell.edu
To compare antibacterial function in macrophages from mice deficient in
the respiratory burst oxidase or inducible nitric oxide
synthase, we developed a fluorescence-based microplate assay of
bacterial survival. iis bacteria grow, they convert a
formulation of resazurin termed AlamarBlue from its nonfluorescent
oxidized state to its fluorescent reduced state, The time
required to achieve a given fluorescence is inversely proportional to
the number of viable bacteria present when the dye is
added. This relationship allows a precise, accurate assessment of
bacterial numbers with greater sensitivity and throughput and
at less cost than conventional assays. The assay facilitated
quantification of the killing of Escherichia coli by
S-nitrosoglutathione
and hydrogen peroxide and of Salmonella typhimurium by human neutrophils
and mouse macrophages, Mouse macrophages
lacking the 91-kDa subunit of the respiratory burst oxidase were
deficient in their ability to kill S. typhimurium, while those
lacking inducible nitric oxide synthase were unimpaired.
