CMV-antigenemia using flow cytometry

Burki Burki at amm.unibe.ch
Fri Dec 12 15:23:36 EST 1997


thanks for your comment. I would be interested in your background and
possibly in your protocol.

We tried PCR, too. We found a sensitivity inferior to the ones obtained
by shell vial or antigenemia. We will continue trying PCR with a
modified  protocol to push sensitivity although major obstacles do not
permit introduction of PCR-assays for this question:
- limited number of assays in our hospital, justifying 1 or 2
runs/week   only
- limited funding; insurances are reluctant to pay 2-3 times more for
a   method which is not significantly better.



cyc wrote:
> we do CMV PCR for routine tests,  results in 2 days.

Didi Burki, Berne/Switzerland
(to reply please replace amm by imm)

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