Hi Maria,
A couple of points.
In my opinion Pseudomonas is a name given to G- non-sporeforming bacteria,
when their biochemical tests do not place them in any particular coherent
grouping. Its a gross generalisation but take a look at bergeys manual and
the number of disparate biochemical tests that you need to do to identify them
and you will see what i mean. In saying this Pseuds are well known for having
plasmid encoded pathways for breaking down a range of chloro-phenolic and
other recalcitrant organic compounds. So if this is what you bug does and is
G- then you could possibly characterise it as a presumptive Pseud.
Gram staining these are that will decide what API id kits you may need. There
are also a number of differential agars from oxoid and similar companies that
will give you more of an idea what they are. I can't think of any specific
ones for Pseuds but at least it will give you an idea of what it isn't. For
exaple McConkey identifys PRESUMPTIVE coliforms. Presumptive because you need
a range of other tests to confirm.
Alternatively, if you have a friendly molecular biologist who works on 16s RNA
based identifications. By a process of PCR of the 16s RNA region, cloning and
subsequent DNA sequencing, the bugs can be placed in particular phylogenetic
groups of related organisms.
Alternatively #2 There is a method called pyrolysis mass spectormetry which
can rapidly characterise bug by their pyrolysed fatty acid profiles. Bugs are
then identified by comparison to neural network of info on other bugs. Have a
look at the www page below for more info
http://gepasi.dbs.aber.ac.uk/roy/pymshome.htm
Hope this helps
Ronan
In article <89ED47C52 at server1.rtc-athlone.ie>, mdavoren at SERVER1.RTC-ATHLONE.IE
("MDAVOREN") wrote:
>Hi All,
>>I am currently working with microbial cultures that are cabable of
>breaking down a certain chemical I work with. I know that I have a
>mixed culture as plating out has revealed several different types of
.........
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Dr. R O'Kennedy
The Advanced Centre for Biochemical Engineering,
Department of Chemical and Biochemical Engineering,
University College London,
Torrington Place,
London WC1E 7JE
e-mail r.o'kennedy at ucl.ac.uk
phn 0171 419 3247
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