IUBio

beta-lactamase: how to define a new enzyme

Karl Fischer tyr-2 at bones.biochem.ualberta.ca
Wed Jul 24 17:30:23 EST 1996


In article <aottolen.43.31F61539 at postbox.acs.ohio-state.edu>,
aottolen at postbox.acs.ohio-state.edu (Abramo Ottolenghi) wrote:

> In article <4t2rkf$dps_001 at du.hku.hk> lksiu at hkucc.hku.hk (lksiu) writes:
> 
> >Subject: beta-lactamase: how to define a new enzyme
> >
> 
> 
> >I am a postgraduate student. I am working with the beta-lactamase research. 
> >Now I have got a problem in naming a beta-lactamase. I have sequenced a
lactamase gene.
> >I found that there is a point mutation within the lactamse gene encoded
region. 
> >Amino change form arg to gly was obtained. However, no change of
isoelectric focusing 
> >point to the parental enzyme was found. Substrate and inhibition
profile was also 
> >similar to the parental enzyme. Thus, could I call this gene as the
parental gene?
> >If I cannot call this gene as the parental beta-lactamase then how to
name this 
> >mutated gene?


Since the beta-lactamase is "wild-type" for all the parameters tested, why
not just name it lactamase type R(amino acid number)G - eg. TEM-1 R283G?

Just my 2¢ worth.

Karl the hepB guy

-- 
Karl Fischer
tyr-2 at bones.biochem.ualberta.ca





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