IUBio

Assay for Carbon 14

Richard J Treinen rtreinen at arco.com
Wed Jul 24 08:58:51 EST 1996


I'm looking for sources of a C-14 assay often used with biological
samples.  I've used this technique 20 years ago, however, I can't
remember the equipment brand name.   The name Carboxysorb comes to mind -
however I've had no luck searching under this name.  The technique
involves a total oxidation of biologic tissue sample.  The produced CO2
is trapped in a fluid and flushed into a scintilation vial ready for
counting.  The method worked well with liver tissue.  We identified the
binding sites of metabolized carbontetrachloride.  The equipment I used
then and am looking for now was automated.    A weighed sample was placed
in the oxidation chamber, a button was pushed, all the carbon was burned
to CO2 which was trapped in a fluid ready for scintilation counting.  My
current application of this method is for tracer analysis of crude oil
samples.  Direct scintilation counting of crude oil does not work due to
the opaqueness of the crude oil.  Is anyone familar with the equipment
used for this assay?  I would appreciate some leads.

Richard Treinen
                       rtreinen at arco.com
            Arco Exploration & Production Technology
                          Plano, Texas



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