On 18 Jul 1996, ELIZABETH PRESCESKY wrote:
> Hello,
> I was wondering if anyone had a procedure for staining marine
> bacteria from water samples using DAPI.
> Thanks in advance,
> Joy
>Dear Joy:
I want to give you the following references about
Epifluorescents counting bacteria from water samples:
1. American Society for Testing and Materials. 1985. Standard
Fluorescence Microscopy Counting Procedure. Publication D4455-85, Vol.
11.02 Water (II). Pages 632-935. American Society for Testing and
Materials, Philadelphia. >
2. KEPNER, R.L. and PRATT, J.R. 1994. Use of Fluorochromes
for Direct Enumeration of Total Bacteria in Environmental Samples: PAst
and Present. Microbiol. Rev., Vol. 58, No.4, pages 603-615.
In the last reference, you are going to find plenty information about
DAPI staining.
Hope this is helpful.
Sincerely,
Fanny Lucia Devia.
Research microbiologist at ICP.
Colombia.
