Spheroplasting Bacillus sp.

Martin J Gill m.j.gill at bham.ac.uk
Thu Jul 18 09:18:56 EST 1996

Could anyone help us by sending me methods for spheroplasting B. cereus. 
We have tried the standard lysozyme/sucrose methods but are either not
'plasting' or are allowing them to burst!  Any personal experience of particular 
problems would be very helpful.
Martin Gill

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