I am just getting into growing anaerobes in an anaerobic chamber and
have read all the literature I can find, but still have many questions.
The media recipes I find say to boil and gas the media with CO2/N2
before autoclaving.
Do I just hang a tube attatched to the gas supply over the edge of a
flask into the air above the media and put the flask over a bunsen
burner?
Do I need something special at the end of the tube?
Do I need a special type of tubing or will ordinary PVC work?
Do I need to get a round-bottom flask or can I use an Ehrlenmeyer?
Can I use a Bunsen burner or must I use a heating mantle?
What is the simplest way to seal the flask in the autoclave?
Can I just skip the gases and put Oxyrase in the media?
Would simply putting media with a reducing agent into the chamber cause
it to become anaerobic? I know that is what the clinical labs do with
their plates, but I don't know if it would work for liquid.
