Nitin S. Baliga wrote:
>> It's becoming a rather well known fact that PCR products are difficult to
> clone. I wonder if anyone has found out ways to get around this problem,
> since PCR is a very powerful technique, and use of this technique may get
> limited to analytical research if cloning continues to be a problem!
> Nitin
We are quite successful in cloning PCR fragments by using PwoI
polymerase. The basic protocol involves amplification, kinase treatment
(if vector treated with alkaline phosphatase, and then ligation.
--
Miguel A. Valvano, M.D., Associate Professor,
Department of Microbiology and Immunology
University of Western Ontario
London, Ontario, N6A 5C1, Canada
Phone (519) 661-3996/3433
Fax (519) 661-3499
