Hi,
You can refer to the paper published from our lab in J.Bact(Jul 1990),
p.4118-4121, where primer extension has been used to estimate levels of
mRNA. You will have to first normalise the values of cDNA synthesis
for different samples, either by using a control gene expression or by
finding the relationship between cDNA synthesis and abundance of mRNA in
the reaction mixture (The 2 should be directly proportional).
Nitin
On Fri, 28 Jun 1996, Hui Zhao wrote:
> Hi:
> Is there any one that ever tried to use RT-PCR or in situ
> hybridization to detect mRNA level from bacteria? Any information is
> appreciated.
>> hui zhao
> university of maryland school of medicine
>>>
