RT-PCR in bacteria

username at BBSRC.AC.UK Your Name Here Your
Mon Jul 1 12:51:10 EST 1996

In article <Pine.A32.3.91.960628142615.14597B-100000 at umabnet.ab.umd.edu>, Hui Zhao <hzhao at umabnet.ab.umd.edu> says:
>        Is there any one that ever tried to use RT-PCR or in situ 
>hybridization to detect mRNA level from bacteria? Any information is 
>hui zhao
>university of maryland school of medicine
Promega kit ACCESS RT-PCR will work with bacterial RNA. I isolate using the Aiba hot phenol method.
First strand synthesis with the Pharmica cDNA cloning kit will also work.
The only problem you may have is too many bands but you just have
to play around with primers and annealing temps.
Good luck

Greer Wilson

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