In article <Pine.A32.3.94.961217145127.57277A-100000 at umabnet.ab.umd.edu>
Doug Stemke <dstemke at umabnet.ab.umd.edu> wrote:
> Hello all.
>> I've been asked to screen for promoters from our
> methano/therophilic Bacillus species. I'd like to screen first in
> E.coli and B.subtilis but currently don't have access to any useful
> vectors, maybe a promoterless CAM for the subtilis and a promoterless lac
> system for E.coli. I would appreciate any commercial vectors or the
> name of any vectors I can order from the Bacillus Stock Culture or ATCC.
>> Thanks in advance for any info.
>> Doug
>> _______________________________________________________________________
>> Douglas J. Stemke, Ph.D
> Biological Process Technology Institute
> University of Minnesota
> St. Paul, MN 55113
Check out Pharmacia's catalogue, they have promoterless lac systems
Sean
