I have a question about GTG start codons, which we find from
time to time in P. aeruginosa (and other Pseudomonads, I assume). I've
never done an N-terminal protein microsequence to see whether the
first amino acid was a Valine (what you'd expect from GTG) or whether
the bug mis-reads the GTG at the N-terminus and puts an f-Met there
instead. If you know, please reply here or by email--I want to be sure
to submit the correct protein translation to the databases. Thanks,
Bruce Ritchings, University of Florida
