I'm using my brothers acc at the moment but I an be contacted at
ronan.okennedy at ucg.ie
Off the top of my head two things come to mind. One have you tried
growing on reduced agar conc. plates (eg <1%) They may be a bit
difficult to spead onto but it should be able to resuspend single
colonies relatively easily. The other thing I'm thinking is that cell
size within colonies of bacteria (esp streptomyces) can be extremely
hetrogenous . OD measurements are sensitive to varations in cell size
so that measurements under one set of conditions (that used to set up a
standard curve) may not be applicable to other growth conditions.
Thats what I can think of at the moment. Get back to me if you want to
talk about it
regards
Ronan O'Kennedy
Dept of Microbiology
University College Galway
Ireland
ronan.okennedy at ucg.ie
or ronan.okennedy at frmmic.ucg.ie
