lysis of Ecoli

J. Dennis Pollack pollack.1 at osu.edu
Thu Dec 5 09:11:39 EST 1996

Help! I need a lysis technique to release cytoplasmic enzymes from transformed 
Ecoli. I cant denature the proteins and hence cant use some of the more 
frequently advertised techs. I suspect lysozyme will work, but I dont want to 
sonicate if I can avoid it. The procedures in Maniatis or Current Protocols 
are not detailed enough. Can anyone help? Thanks a lot. Dennis

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