PGal concentration

André Gallant soniag at nbnet.nb.ca
Wed Dec 4 19:27:06 EST 1996

Hi everyone,

I've been trying to use a phenyl-B-D-galactoside (PGal) medium to select
lacI mutants (even tried wt E.coli with IPTG to test the medium), but I
haven't observed any growth.  Is it possible that my concentration of
PGal is too high (750mg/L)?
Andre Gallant
grad student
Dept Chimie-Biochimie
Universite de Moncton
E-mail:  soniag at nbnet.nb.ca

"If I knew what I was looking for...It wouldn't be called research"

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