Arul Jayaraman <arul at design.eng.uci.edu> wrote:
>hi
>Can anyone give me a reference where the MPN test is described in detail?
>Thanks for your assistance
>Arul
>---------------------------------------------------------------
>Arul Jayaraman
>Dept. of Chemical & Biochemical Engineering
>University of California at Irvine
>Irvine, CA 92697-2575
>Ph : (714)-509-1060 (H)
> (714)-824-8389 (L)
>http://www.eng.uci.edu/~arul/
>
After modeling data with the equations associated with the MPN
technique, it seems to me that this technique is useful only in the
circumstance where a reasonable guess may be made concerning the cell
density, so that dilution steps of only 1:3, 1:4, or 1:5 can be
employed, with or without an initially large dilution. This enables a
good "bracketing" of the MPN. Dilution steps of 1:10 are hardly worth
it in terms of quality of the result. Therefore, I wouldn't recommend
the technique for general use, despite the fact that it is often
recommended by others.
