Reference for Most Probable Number (MPN) test wanted

Bob Presswood rpressw at ix.netcom.com
Tue Dec 3 22:30:57 EST 1996

Arul Jayaraman <arul at design.eng.uci.edu> wrote:

>Can anyone give me a reference where the MPN test is described in detail?
>Thanks for your assistance

>Arul Jayaraman
>Dept. of Chemical & Biochemical Engineering
>University of California at Irvine
>Irvine, CA 92697-2575
>Ph : (714)-509-1060 (H)
>     (714)-824-8389 (L)


After modeling data with the equations associated with the MPN
technique, it seems to me that this technique is useful only in the
circumstance where a reasonable guess may be made concerning the cell
density, so that dilution steps of only 1:3, 1:4, or 1:5 can be
employed, with or without an initially large dilution.  This enables a
good "bracketing" of the MPN.  Dilution steps of 1:10 are hardly worth
it in terms of quality of the result.  Therefore, I wouldn't recommend
the technique for general use, despite the fact that it is often
recommended by others.

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