pH Measurement of Small Biological Samples

Roy Markussen roy at miclis.no
Mon Dec 2 14:38:05 EST 1996

Steven Lemore wrote:
> The need frequently arrises in biological laboratories to measure pH in
> small sample vessels such as 96 well plates, microcentrifuge tubes, or
> NMR tubes.  Standard pH electrodes are too large to fit into these small
> containers. Miniature glass electrodes are both breakable and have a high
> electrical resistance which makes the pH measurements unstable. A micro
> combination pH electrode has been developed which has a 1 mm tip and a 2
> mm diameter flexible Teflon body which can measure pH in less less than
> 20 microliters of sample. This probe has a solid state sensor which gives
> drift free pH measurements. The probe can be used with a standard pH
> meter. Further information can be obtained via email at
> service at lazarlab.com


I would use an IFSET electrode, i.e. from Sentron. These electrodes only
requires one drop of the sample, and the response time is brilliant.

If you absolutley have to dip the electrode _in_ then sample, I'm out of

Roy Markussen
Tonsberg Public Food Control, Norway
nmt-tonsberg at miclis.no

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