IUBio

Stability of Amplicillin

benedik at uh.edu benedik at uh.edu
Thu Sep 28 17:20:24 EST 1995


In article <44e3br$gvl at merlion.singnet.com.sg>
anomie at singnet.com.sg (Anomie) writes:

> Hello!
> I'm doing a project on charactrerisation of B-lactamases. Recently, we tried to do enzyme kinetics using amplicillin as substrate. However we found out that amplicillin has a very high autohydrolysis rate. Can anyone out there provide me with the ane
>  on how to improve the stability of the antibiotic?
> Thanks

Make it fresh, or store it frozen. If you are doing enzyme assays I
would advise making it fresh. It is very cheap and only takes a minute
or two in order to way some out for fresh substrate.


Michael Benedik               benedik at uh.edu
Biochemical Sciences
University of Houston
Houston, TX 77204-5934



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