In article <44e3br$gvl at merlion.singnet.com.sg>
anomie at singnet.com.sg (Anomie) writes:
> I'm doing a project on charactrerisation of B-lactamases. Recently, we tried to do enzyme kinetics using amplicillin as substrate. However we found out that amplicillin has a very high autohydrolysis rate. Can anyone out there provide me with the ane
> on how to improve the stability of the antibiotic?
Make it fresh, or store it frozen. If you are doing enzyme assays I
would advise making it fresh. It is very cheap and only takes a minute
or two in order to way some out for fresh substrate.
Michael Benedik benedik at uh.edu
University of Houston
Houston, TX 77204-5934