In article <446q6v$pul at morgoth.sfu.ca>,
Julie Wasylnka <wasylnka at news.sfu.ca> wrote:
>I would like to know the growth conditions of mycobacterium smegmatis. I will be using a fermentor to grow these cultures. Thanks for any information.
>>Julie Wasylnka
>Institute of Molecular biology and biochemistry
>Simon Fraser University
>Burnaby, B.C.
>>In the lab, we usually grow. M. smegmatis on Middlebrook's 35. This might
be a little expensive for a fermenter. In a pinch, I've used Luria broth
and gotten growth. We use shaking at 37 degrees C. Good luck
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