non-radioactive marker enzyme assays

Torsten Boerchers borcher at uni-muenster.de
Sat Sep 23 12:46:39 EST 1995

Is anybody aware of a nonradioactive method
to determine the enzymes that confer antibiotic
resistance, in particular vs.kanamycin or hygromycin?
Those enzymes are  phosphotransferases (nptII, hpt) and 
inactivate the antibiotics by phosphorylation.I am looking
for an alternative to the thin layer chromatography
with subsequent autoradiography.

Furthermore, does anybody know to which amount these
enzymes are actually expressed in the transgenic plants?
Can they be seen in Western Blotting and are antibodies

Any hints in the right direction are highly appreciated.
                          Torsten Boerchers                            /
    _/  _/_/_/ _/_/_/    Institute of Chemical- and Biochemical Sensor/
   _/  _/     _/   _/   Research (Molecular Biology Group)           /
  _/  _/     _/_/_/    Mendelstr 7, D-48149 Muenster, Germany       /
 _/  _/     _/    _/  Fax: +49-251-980 2890 Phone: +49-251-980 2876/
_/  _/_/_/ _/_/_/_/  E-Mail: borcher at uni-muenster.de              /

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