I'm doing a project on characterisation of B-lactamases. We're using Cephalosporin C as a substrate in the kinetic assay. At the moment we're facing the problem of not being able to dissolve Cephalosporin C in 0.025M phosphate buffer at pH7.
Anybody who could help us to solve this problem please reply because if this is not solve, I can't continue with the project which will be due next year.
Thank a lot.
PS: The last time, we 're trying to dissolve 0.02g of cephalosporin in 100ml of 0.025m phosphate buffer at pH 7