catechol 1,2-dioxygenase

bobo sakuragi at unixg.ubc.ca
Mon Sep 18 20:41:17 EST 1995

I'm trying to assay for dioxygenase activity in Streptomyces (C12O, C23O, 
PA34O, and PA45O) but I've been having some problems.  First, I don't 
have controls for ortho cleaving enzymes.  Second, the spectrophotometric 
method that I've been using for catechol 1,2-dioxygenase activity 
(i.e. monitor for cis-cis-muconic acid formation at A260) hasn't been 
working out due to the fact that I've been working with very crude 
extracts.  I found a colorimetric assay by K. Hosokawa which apparently 
results in the formation of a purple color.  Does anyone have any 
experience with these assays?   Also if anyone knows of or has any 
Streptomyces or Pseudomonas (thought I'd prefer Streptomyces) isolates 
that have ortho-cleavage activity I could really use them.  If you have 
any information please feel free to e-mail me:

Sakura Grace Iwagami
Dept of Microbiology
University of British Columbia
Vancouver, Canada

sakuragi at unixg.ubc.ca
sakura at davies.microbiology.ubc.ca

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