In article <41ndui$he6 at ixnews2.ix.netcom.com>, chicago at ix.netcom.com (Chicago) writes:
> In <mbransde-080895165122 at mpg7-97.appbio.utas.edu.au>
>mbransde at postoffice.newnham.utas.edu.au (Matthew Bransden) writes:
>> For universal PCR primers or species specific primers, the 16S rRNA gene
is obviously the best characterised and most likely candidate.
For universal, compare sequences of widely unrelated bugs, on say Genbank.
Species specific 16S- rRNA sequences should be under orgs. name. Design your
own primers.
In my experience with mycoplasma, this approach works well. Choose a few primer
pairs to find the most efficient combination(s).
Yours,
John
