In article <1995Oct9.181237 at ania.path.ox.ac.uk>, sbaker at molbiol.ox.ac.uk (Simon Baker) says:
>>I am looking for a method to transform Pseudomonas species.
>I'm aware of the electroporation methods, but if there are
>any "calcium-chloride-like" protocols, I'd be very interested
>to hear of them
Simon,
Have a look at Steward & Carlson (Annu. Rev. Microbiol. 40:211 (1986)).
I don't have the article to hand at the moment, but I think it might
help.
Vince Mulholland
Scottish Agricultural Science Agency.