Microwaves: how effective at sterilization

Dr. Michael J. Miller mignatz at mjmiller.roc.servtech.com
Wed Oct 18 18:54:28 EST 1995

In article <Pine.SGI.3.91.951018232511.29498A-100000 at tower.york.ac.uk>, RM
Brownlie <rmb10 at york.ac.uk> wrote:

> Recently when in a rush for LB plates I have resorted to 
> microwaving 100 ml quantities and have found these to be clean every time. 
> Does any one know if this is a very reliable method for sterilization. 
> Does it inactivate spores and is sterilization dependant on an aqueous 
> environment? Could it be used to sterilize plasticware for instance.
> Thanks for any response
> Robert 

Actually, there are a few companies that use microwaves to sterilize small
quantities of media (unfortunately, I can't remember their names).  You
put the media into small containers, the containers are sealed, and then
microwaved.  The increase in pressure in the small containers will elevate
the temperature, hence, an autoclave-like setting.  I don't believe anyone
is using microwaves to sterilize plastics, however.  Good luck!


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