C. perfringens viability and cell counts

Keith Warner kwarner at unccvm.uncc.edu
Sat Oct 7 00:34:18 EST 1995

Can anyone tell me if it is possible to obtain reliable cell counts for 
C. perfringens.  I would like to conduct heat stress studies, but I'm 
finding that spread plating and spot plating do not produce consistent 
numbers.  What suggestions do you have?  I am considering using a 
fluoresent staining procedure to count cells directly, but would like to 
stick with the plates.  Any advice would be greatly appreciated.

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