how to kill B. subtilis spores?

Forday Wayne Lee fwl at titan.np.ac.sg
Thu Nov 30 19:43:14 EST 1995

In article <49k29s$gk2 at chaos.kulnet.kuleuven.ac.be>,
chris michiels  <chris.michiels at agr.kuleuven.ac.be> wrote:
:>We started recently working with B. subtilis spores, and have to plate 
:>serial dilutions frequently. For vegetative bacteria, this is done with a 
:>Drigalski spatula, that can be easily sterilized by immersion in ethanol. 
:>However, B. subtilis spores are not killed in this way. Is there another 
:>way to efficiently sterilize the spatula's (like chlorine?), or is 
:>autoclaving the only safe solution?

Have you tried hydrogen peroxide or peracetic acid?

Wayne Lee Forday, Biotechnology Department 
Ngee Ann Polytechnic, Singapore 
fwl at np.ac.sg

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