We started recently working with B. subtilis spores, and have to plate
serial dilutions frequently. For vegetative bacteria, this is done with a
Drigalski spatula, that can be easily sterilized by immersion in ethanol.
However, B. subtilis spores are not killed in this way. Is there another
way to efficiently sterilize the spatula's (like chlorine?), or is
autoclaving the only safe solution?
Thanks for any suggestions.
Chris Michiels
